ELISA/assay

ANTI BETA2 GLYCOPROTEIN 1 IgM ELISA

DCM111

1 x 96 well

415 USD

ANTI BETA2 GLYCOPROTEIN 1 IgM ELISA

Anti Beta 2 Glycoprotein 1 IgM ELISA Assay Kit is based on the initial binding of the antibodies present in calibrators, controls or pre-diluted samples to the beta 2 Glycoprotein 1 coated on the inner surface of the microplate wells. After a 60 minutes incubation the microplate is washed with a wash buffer to remove the non-reactive serum components.  Then an anti-human-IgM horseradish peroxidase conjugated solution recognizes IgM class antibodies bound to the immobilized Antigenss. After a 30 minutes incubation the excess of enzyme conjugate, which is not specifically bound, is washed away with the wash buffer.  Finally, a chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation color development is stopped by adding the stop solution. The solution turns yellow at this point. The level of color is directly proportional to the concentration of IgM antibodies present in the original sample. The concentration of IgM antibodies present in the sample is calculated through a calibration curve.

ELISA

Assay Kits

Immunology

1 x 96 well

ANTI-BETA2_GLYCOPROTEIN

ANTI-BETA2 GLYCOPROTEIN, Beta, 2, anti, IgM

0.11 AU/mL

10 - 160AU/mL

10 ul

Serum, Plasma

2 hours

Human

RUO

wet

2-8C

415 USD