ELISA/assay

Anti-Tissue Transglutaminase (tTG) IgG ELISA Assay Kit

DCM109

1 x 96 wells

565 USD

Anti-Tissue Transglutaminase (tTG) IgG ELISA Assay Kit

Anti Tissue Transglutaminase IgG test is based on the binding of serum or plasma antibodies on the human recombinant tissue transglutaminase coated into the microplates. The antibodies in calibrators, controls or prediluted patient samples bind into the inner surface of the wells. After a 30 minutes incubation the microplate is washed with wash buffer for removing non-reactive serum components. An anti-human-IgG horseradish peroxidase conjugate solution recognize IgG class antibodies bound to the immobilized antigens. After a 30 minutes incubation any excess enzyme conjugate, which is not specifically bound is washed away with wash buffer. A chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the color development is stopped by adding the stop solution. The solutions color change into yellow. The amount of color is directly proportional to the concentration of IgG antibodies present in the original sample.

ELISA

Assay Kit

Immunology, Gastroenterology

1 x 96 wells

0.12 AU/mL

5 - 320 AU/mL

10 uL

Serum, Plasma

1 hour 15 minutes

Human

RUO

wet

2-8C

565 USD