ELISA/assay

Anti-Tissue Transglutaminase (tTG) IgA ELISA Assay Kit

DCM108

1 x 96 wells

590 USD

Anti-Tissue Transglutaminase (tTG) IgA ELISA Assay Kit

Anti Tissue Transglutaminase IgA test is based on the binding of serum or plasma antibodies on the human recombinant tissue transglutaminase coated into the microplates. The antibodies in calibrators, controls or prediluted patient samples bind into the inner surface of the wells. After a 30 minutes incubation the microplate is washed with wash buffer for removing non-reactive serum components. An anti-human-IgA horseradish peroxidase conjugate solution recognize IgA class antibodies bound to the immobilized antigens. After a 30 minutes incubation any excess enzyme conjugate, which is not specifically bound is washed away with wash buffer. A chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the color development is stopped by adding the stop solution. The solutions color change into yellow. The amount of color is directly proportional to the concentration of IgA antibodies present in the original sample.

ELISA

Assay Kit

Immunology, Gastroenterology

1 x 96 wells

0.11 AU/mL

5 - 320 AU/mL

10 uL

Serum, Plasma

1 hour 15 minutes

Human

RUO

wet

2-8C

590 USD