ELISA/assay

ANA Screen ELISA Assay Kit

DCM099

1 x 96 wells

455 USD

ANA Screen ELISA Assay Kit

The ANA Screen test is an indirect enzyme immunometric assay (ELISA) based on the binding of present antibodies with Sm, RNP/Sm, SS-A (Ro), SS-B (La), Scl-70, Jo1, U1-SmRNP, CENP-B, dsDNA and Histones antigens coated on the microplates. Any antibodies present in calibrators, controls or prediluted patient samples bind to the inner surface of the wells. After a 30 minutes incubation the microplate is washed with wash buffer for removing non-reactive serum components. An anti-human-IgG horseradish peroxidase conjugate solution recognises IgG class antibodies bound to the immobilised antigens. After a 30 minutes incubation any excess enzyme conjugate, which is not specifically bound is washed away with wash buffer. A chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the colour development is stopped by adding the stop solution. The solution turns yellow at this point. The level of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

ELISA

Assay Kit

Immunology

1 x 96 wells

10 uL

Serum, Plasma (heparin or EDTA)

1 hour 15 minutes

Human

RUO

wet

2-8C

455 USD