ELISA/assay

Anti-dsDNA IgG ELISA Assay Kit

DCM095

1 x 96 wells

455 USD

Anti-dsDNA IgG ELISA Assay Kit

Anti dsDNA IgG test is based on the binding of serum or plasma IgG antibodies on dsDNA coated on the microplates. In the first step the antibodies in calibrators, controls or prediluted patient samples bind into the inner surface of the wells. After an incubation the microplate is washed with a wash buffer for removing non-reactive serum components. In the second step an anti human IgG horseradish peroxidase conjugated solution recognizes the IgG class antibodies bound to the immobilized dsDNA antigens. After an incubation any excess of enzyme conjugate, which is not specifically bound, is washed away with the wash buffer. Then a chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the color development is stopped by adding the stop solution. The solution color changes into yellow. The amount of color is directly proportional to the concentration of the Anti dsDNA IgG antibodies present in the original sample. The concentration of the anti dsDNA IgG antibodies in the sample are calculated through a calibration curve

ELISA

Assay Kit

Immunology

1 x 96 wells

0.135 IU/mL

12.5 - 200 IU/mL

10 uL

Serum, Plasma

1 hour 45 minutes

Human

RUO

wet

2-8C

455 USD