ELISA/assay

Human VDBP (GC Globulin) ELISA Assay Kit

DBP31-K01

1 x 96 well

920 USD

Human VDBP (GC Globulin) ELISA Assay Kit

The Eagle Biosciences Human VDBP (GC Globulin) quantitative VDBP ELISA Assay Kit is a solid phase competitive immunoassay designed to detect VDBP. Microwells are coated with anti-VDBP antibody. Assay calibrators, control and diluted unknown serum or plasma specimens are added to the microwells along with GC Globulin HRP conjugated antibody. After an incubation period, the immunocomplex of solid phase bound “VDBP Antibody-VDBP” is formed, which inhibits the formation of “VDBP Antibody – HRP Conjugated VDBP”. Unbound HRP Conjugated VDBP are removed with a washing step. During a second incubation with TMB substrate, a blue color is developed. The enzyme-substrate reaction is stopped by the addition of sulfuric acid. The absorbance of assay calibrators, controls and unknown specimens are measured by an ELISA plate reader with wavelength set at 450 nm. The color intensity is inversely proportional to the amount of VDBP present in the calibrators controls and specimens.

ELISA

Assay Kits

Vitamin Assays

1 x 96 well

Vitamin D

VDBP Vitamin D Binding Protein

0.0637 µg/mL

0 - 20.1 µg/mL

10 µL

Serum, Plasma

1.5 hours

Human

RUO

wet

2-8C

920 USD