ELISA/assay

Hamster (CHO) CTSA ELISA Kit

CTS91-K01

96 wells

1420 USD

Hamster (CHO) CTSA ELISA Kit

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Lysosomal Protective Protein (CTA) present in samples reacts with the anti-CTA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-CTA, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CTA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CTA in the test sample. The quantity of CTA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay Kit

Immunology

96 wells

Lysosomal Protective Protein

Lysosomal Protective Protein

0.3125 - 20 ng/mL

100 uL

Lysate, Supernatant Samples

2 hours 50 minutes

Hamster

RUO

wet

2-8C

1420 USD