ELISA/assay

Complement TCC ELISA Assay Kit

COMPL TCC

1 x 96 well

980 USD

Complement TCC ELISA Assay Kit

The assay is a colorimetric sandwich ELISA. Samples are diluted in assay diluent and 100uL diluted sample is transferred to the microtiter wells and incubated at room temperature for 60 minutes. During this first incubation TCC in the sample is captured by the anti-TCC monoclonal antibody, pre-coated on the surface of the microtiter wells. After washing to remove unbound material, a second horseradish peroxidase (HRP) labelled monoclonal antibody is added to detect the TCC bound to the well. After incubation for 30 minutes the wells are washed again and a substrate is added and incubated. The color development is stopped after 30 minutes and the color is measured in a spectrophotometer. The color is directly proportional to the amount of TCC bound to the well. The amount of TCC is determined by comparison to the color development of the calibrator samples.

ELISA

Assay Kits

Immunology

1 x 96 well

sTCC

sTCC, soluble terminal complement complex

3 ng/mL

10 - 400 ng/mL

30 uL

EDTA Plasma

2 hours

Human

RUO

wet

2-8C

980 USD