ELISA/assay

cAMP Urine ELISA Assay Kit

CMP14-K01

1 x 96 wells

340 USD

cAMP Urine ELISA Assay Kit

The cAMP Urine ELISA is a competitive immunoassay. Competition occurs between cAMP present in calibrators, controls, specimen samples and an enzyme-labeled antigen (HRP conjugate) for a limited number of anti-cAMP antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of cAMP present. Following an incubation, the enzymatic reaction is terminated by the addition of the stopping solution, converting the color from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of cAMP in specimen samples and controls can be directly read.

ELISA

Assay Kit

Endocrinology, Neurology

1 x 96 wells

cyclic adenosine-3', 5'-monophosphate, cAMP

cyclic adenosine-3', 5'-monophosphate, cAMP

0 - 300 pmol/mL

100 uL

Urine

1 hour 20 minutes

human

RUO

wet

2-8C

340 USD