ELISA/assay

Fecal C. Difficile Toxin AB Qualitative ELISA Assay Kit

CDT35-K01

1 x 96 well

455 USD

Fecal C. Difficile Toxin AB Qualitative ELISA Assay Kit

This Eagle Biosciences Fecal C. Difficile Toxin A & B ELISA Assay Kit is a “sandwich” ELISA is designed, developed and produced for the qualitative measurement of Toxin A and Toxin B in stool specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter wells.  Controls and extracted fecal specimen are added to microtiter wells of microplate that was coated with a highly purified monoclonal anti-Toxin A and Toxin B on its wall. During the assay, the Toxin A and B Antibodies will be bound to the antibody coated plate after an incubation period. The unbound material is washed away and another HRP-conjugated monoclonal antibody which specifically recognizes the protein of Toxin A & B is added for further immunoreactions.  After an incubation period, the immunocomplex of “Anti-Toxin A & B Capture Antibody – Toxin A & B – HRP-conjugated Anti-Toxin A & B Tracer Antibody” is formed if Toxin B is present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to Toxin A & B proteins captured on the wall of each microtiter well is directly proportional to the amount of Toxin A & B level in each test specimen.

ELISA

Assay Kits

Gastroenterology

1 x 96 well

Clostridium Difficile Toxin A & B

Clostridium Difficile Toxin A & B

Cut-off

Cut-off

250 mg

Stool

2 hours

Human

RUO

wet

2-8C

455 USD