ELISA/assay

Canine Cystatin C ELISA Assay Kit

CCE41-K01

96 wells

685 USD

Canine Cystatin C ELISA Assay Kit

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Cystatin C (CYS) present in samples reacts with the anti-CYS antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti- CYS is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CYS in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CYS in the test sample. The quantity of CYS in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.

ELISA

ASSAY kit

Veterinarian, Immunology

96 wells

Cystatin C

Cystatin C

0.07 ng/mL - 4 ng/mL

102 μL

Plasma, Serum, Urine

1 hour 50 minutes

Dog

RUO

wet

2-8°C

685 USD