ELISA/assay

Human Trail Receptor 1/DR4/CD261 ELISA Assay Kit

C6131-K01

1 x 96 well

665 USD

Human Trail Receptor 1/DR4/CD261 ELISA Assay Kit

A capture Antibody highly specific for the Eagle Biosciences TRAIL R1 has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of TRAIL R1 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed. During the next incubation period the binding of the biotinylated anti- TRAIL R1 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of TRAIL R1 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of TRAIL R1 in any sample tested.

ELISA

Assay Kits

Immunology

1 x 96 well

Trail Receptor 1/DR4/CD261

Trail Receptor 1/DR4/CD261

8 pg/ml

31.25-1000 pg/ml

100 ul

Serum, Cell lysates, Cell culture supernatant

2h30

Human

RUO

wet

2-8C

665 USD