ELISA/assay

Human CD141 / THROMBOMODULIN ELISA Assay Kit

C4131-K01

1 x 96 well

665 USD

Human CD141 / THROMBOMODULIN ELISA Assay Kit

A capture Antibody highly specific for CD141 has been coated to the wells of the microtitre strip plate provided during manufacture. Binding of CD141 samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-CD141 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of CD141 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of CD141 in any sample tested.

ELISA

Assay Kits

Immunology

1 x 96 well

CD141 / THROMBOMODULIN

CD141 / THROMBOMODULIN

0.31 ng/ml

0.625 - 20 ng/ml

100 ul

Serum, Plasma, Cell Culture

1.75 hours

Human

RUO

wet

2-8C

665 USD