ELISA/assay

Mouse C1q ELISA Kit

C1Q21-K01

96 wells

705 USD

Mouse C1q ELISA Kit

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the C1q present in samples reacts with the anti-C1q antibodies, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-C1q antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound C1q . Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of C1q in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of C1q in the test sample. The quantity of C1q in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.

ELISA

ASSAY kit

Immunology, Oxidative Stress

96 wells

C1q

C1q, Complement Component 1 q

1.57 ng/mL - 100 ng/mL

100 μL

Plasma, Serum

1 hour 40 minutes

Mouse

RUO

wet

2-8°C

705 USD