ELISA/assay

Bovine SAA ELISA Kit

BSA61-K01

12 x 8 wells

665 USD

Bovine SAA ELISA Kit

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Serum Amyloid A (SAA) present in samples reacts with the anti-SAA antibodies, which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAA antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay Kits

Immunology

12 x 8 wells

26.34 ng/ml - 300 ng/ml

100 uL

milk/plasma/serum

1 hr 40 mins

Bovine

RUO

wet

4-8C

665 USD