ELISA/assay

Angiopoietin-2 mouse/rat ELISA

BI-ANG2MR

1 x 96 well

725 USD

Angiopoietin-2 mouse/rat ELISA

This kit is a sandwich enzyme immunoassay for the quantitative determination of mouse/rat Angiopoietin-2 inserum and plasma samples. In a first step, STD/CTRL/Sample are pipetted into the wells of the microtiter strips, which are pre-coated with a recombinant monoclonal Angiopoietin-2 antibody. Angiopoietin-2 present in the sample binds to the pre-coated antibody in the well. After a first wash step, which removes non-specifically unbound material, detection antibody is added and forms a sandwich with the antigen bound on the plate. After another washing step the conjugate (streptavidin-HRP) is pipetted into the wells and reacts with the detection antibody. After another washing step, the substrate (TMB, tetramethylbenzidine) is pipetted into the wells. The enzyme catalysed color change of the substrate is directly proportional to the amount of mouse/rat Angiopoietin-2 present in the sample. This color change is detectable with a standard microtiter plate ELISA reader. The concentration of Angiopoietin-2 in the sample is determined directly from the dose response curve. The kit utilizes recombinant mouse Angiopoetin-2 as a calibrator. Mouse, rat, bovine and human Angiopoietin-2 share a high homology (>85%).

ELISA

Assay Kit

Veterinary

1 x 96 well

Angiopoietin-2 , mouse, rat

Angiopoietin-2 , mouse, rat, ANG-2

18.3 pmol/l

0 – 1400 pmol/l

5 uL

Serum, Plasma

5.5 hours

Mouse, Rat

RUO

wet

2-8C

725 USD