ELISA/assay

Soluble Semaphorin 4D ELISA Assay Kit

BI-20405

1 x 96 well

955 USD

Soluble Semaphorin 4D ELISA Assay Kit

This kit is a sandwich enzyme immunoassay for the direct determination of soluble Semaphorin 4D in human plasma samples. In a first step, STD/sample/CTRL are pipetted into the wells of the microtiter strips, which are precoated with anti Semaphorin 4D antibody. Semaphorin 4D present in the STD/sample/CTRL binds to the pre-coated antibody in the well. In the washing step all non-specific unbound material is removed. In a next step, the conjugate (bivalent Fab bacterial alkaline phosphatase fusion antibody-HRP) is pipetted into the wells and reacts with the soluble Semaphorin 4D forming a sandwich. After another washing step, the substrate (TMB Tetramethylbenzidine) is pipetted into the wells. The enzyme catalysed colour change of the substrate is directly proportional to the amount of Semaphorin 4D. This colour change is detectable with a standard microtiter plate ELISA reader. A dose response curve of the absorbance (optical density, OD at 450 nm) versus standard concentration is generated, using the values obtained from the standards. The concentration of Semaphorin 4D in the sample is determined directly from the dose response curve.

ELISA

Assay Kits

Bone Metabolism

1 x 96 well

Bioactive Sclerostin

Bioactive Sclerostin

LOD: (0 pmol/l + 3 SD): 12 pmol/l; LLOQ: 31 pmol/l

62.5 to 2000 pmol/l

10 uL

EDTA plasma, heparin plasma, and citrate plasma

4 hours

Human

RUO

wet

2-8C

955 USD