ELISA/assay

Tryptophan ELISA

BA E-2700

1 x 96 well

830 USD

Tryptophan ELISA

After extraction and derivatization Tryptophan is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standards.

ELISA

Assay Kits

Endocrine

1 x 96 well

Tryptophan

Tryptophan

1.2 µg/ml

2.5 – 250 µg/ml

20 µl

Urine, plasma, serum

Overnight

Human

RUO

wet

2-8C

830 USD