ELISA/assay

Fecal/Tissue Cortisol ELISA

ARG81392

1 x 96 well

975 USD

Fecal/Tissue Cortisol ELISA

This assay employs the competitive enzyme immunoassay technique. A specific anti-mouse-IgG antibody has been pre-coated onto a microtiter plate. Cortisol containing samples or standards and a Cortisol -HRP conjugate are given into the wells of the microtiter plate. Then a mouse monoclonal antibody to Cortisol is added into the wells. Cortisol in samples or standards and a fixed amount of Cortisol -HRP conjugate are competing for a limited amount of Cortisol mouse monoclonal antibody. The Cortisol-antibody complex binds tothe goat polyclonal anti-mouse IgG that has been previously bound to the well. After incubation, the wells are washed with wash buffer to remove unbound material. A substrate solution is then added and incubated, resulting in the development of a blue color. The color development is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured at 450 nm. The concentration of Cortisol is indirectly proportional to the color intensity of the test sample.

ELISA

Assay Kits

Endocrinology

1 x 96 well

Cortisol

Cortisol, Steroid, hormone

17.3 pg/ml

100 - 3200 pg/ml

50 ul

Dried fecal samples, tissue culture media and urine.

1.5 hours

Human

RUO

wet

2-8C

975 USD