ELISA/assay

Human beta-Amyloid (1 - 42) ELISA Kit

ARG81143

1 x 96 well

1,200.00 USD

Human beta-Amyloid (1 - 42) ELISA Kit

Alzheimer’s Disease (AD) is the most common neurodegenerative disorder in elderly people. It has been demonstrated that AD has biological causes and is characterized by the presence of senile plaques and neurofibrillary tangles mainly in cerebral cortex and hippocampus brain regions. Beta-Amyloid (1-40) (Aβ40) and beta-Amyloid (1-42) (Aβ42) are the main components of the above plaques; however, other forms of beta-Amyloid peptides are also present. Both peptides are cleaved from the Amyloid Precursor Protein (APP) by β-secretase and γ-secretase enzymes. Many studies suggest that Aβ42 or/and Aβ43 are required to initiate formation of amyloid plaques and neurofibrills that leads to the neurodegeneration, while Aβ40 is less neurotoxic.

The Eagle Biosciences beta-Amyloid (1-42) ELISA assay kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Aβ 42 has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any Aβ 42 present is bound by the immobilized antibody. After washing away any unbound substances a HRP-labeled Human Aβ (N) monoclonal antibody is added to each well and incubate. After washing away any unbound reagents, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of Aβ 42 bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm. The concentration of Aβ 42 in the sample is then determined by comparing the O.D of samples to the standard curve.

ELISA

Assay Kits

Neurobiology

1 x 96 well

beta-Amyloid (1 - 42)

beta-Amyloid (1 - 42)

0.29 pg/ml

1.56-100 pg/ml

100 µl

EDTA-Plasma, cerebrospinal fluid, cell culture supernatant

Overnight

Human

RUO

wet

2-8C

1,200.00 USD

$1,020