ELISA/assay

Human CRP (High sensitive) ELISA Kit

ARG80876

1 x 96 well

Human CRP (High sensitive) ELISA Kit

C-Reactive Protein (CRP) is an acute-phase protein, produced exclusively in the liver. Interleukin- 6 is the mediator for the synthesis by the hepatocytes of CRP, a pentamer of approximately 120.000 Daltons. CRP is present in the serum of normal persons at concentrations ranging up to 5mg/l. A series of prospective studies provide consistent data documenting that mild elevation of baseline levels of CRP among apparently healthy individuals is associated with higher long term risk for future cardiovascular events. This predictive capacity of CRP is independent of traditional cardiovascular risk factors and offers a prognostic advantage over measurement of lipid alone. Inflammatory markers specifically CRP may help to identify those who would benefit most from these pharmacological intervention. CRP is the novel and evolving biomarker which provides a most useful predictive indicator for subsequent cardiovascular events. This test should not be used for assessment of acute inflammation but should be ordered to evaluate CVD (Cardiovascular Disease) risk in apparently healthy individuals who have not had recent infection or other serious illness.

The Eagle Biosciences High Sensitive CRP assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for CRP has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any CRP present is bound by the immobilized antibody. After washing away any unbound substances, an HRP-CRP antibody conjugate is added to each well and incubate. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of CRP bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450 nm ±2 nm. The concentration of CRP in the sample is then determined by comparing the O.D of samples to the standard curve.

ELISA

Assay Kits

Endocrinology

1 x 96 well

C-Reactive Protein

CRP, C-Reactive Protein

0.02 ug/ml

0.4 - 10 ug/ml

10 ul

serum, plasma

1 hour

Human

RUO

wet

2-8C