ELISA/assay

Human Apolipoprotein H (ApoH) ELISA

APH39-K01

1 x 96 well

630 USD

Human Apolipoprotein H (ApoH) ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Apo-H present in samples reacts with the anti-Apo-H antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Apo-H antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Apo-H. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Apo-H in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Apo-H in the test sample. The quantity of Apo-H in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

ELISA

Assay kit

Cardiovascular

1 x 96 well

Apolipoprotein H (ApoH) ELISA

Apolipoprotein H (ApoH) ELISA

3.125 - 200 ng/ml

100 µl

Biological Fluids

90 minutes

Human

RUO

wet

2-8C

630 USD