ELISA/assay

Adrenaline, Noradrenaline & Dopamine ELISA

AND31-K03

1 x 96 well

Adrenaline, Noradrenaline & Dopamine ELISA

The Adrenaline, Noradrenaline, & Dopamine (Plasma & Urine) ELISA assay kit provides materials for the quantitative measurement of adrenaline, noradrenaline and dopamine in plasma and urine. Noradrenaline, adrenaline and dopamine are extracted using a cis-diol-specific affinity gel and acylated to N-acylnoradrenaline, N-acyladrenaline and N-acyl-dopamine and then converted enzymatically into N-acylnormeta­nephrine, N-acylmetanephrine and N-acyl-3-methoxytyramine.

The competitive Adrenaline, Noradrenaline, & Dopamine (Plasma & Urine) ELISA kit uses the microtiter plate format. Adrenaline, noradrenaline and dopamine, respectively, are bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free Antigens and free Antigens-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.

ELISA

Assay Kits

Catecholamine Kits

1 x 96 well

Adrenaline, Noradrenaline, Dopamine

Noradrenaline, Norepinephine, catecholamine, Adrenaline, epinephine, Dopamine

URINE:  Adrenaline: 0.16 ng/ml, Noradrenaline: 0.85 ng/ml, Dopamine 0.46 ng/ml, PLASMA: Adrenaline: 0.8 pg/ml, Noradrenaline: 43 pg/ml, Dopamine 23 pg/ml

Adrenaline: 0.5 - 250 ng/ml, Noradrenaline: 1.5 - 500 ng/ml, Dopamine:  1.5 - 2560 ng/ml

URINE: 15 ul, PLASMA:  300 ul

Urine, Plasma

Overnight

Human

RUO

wet

2-8C