ELISA/assay

Anti-M2 (Mitochondrial Antibodies) ELISA

AM231-K01

1 x 96 well

Anti-M2 (Mitochondrial Antibodies) ELISA

The group of primary autoimmune liver disease (PAL) comprises autoimmune hepatitis (AIH), PBC and primary sclerosing cholangitis (PSC). The clinical picture of PAL is in most cases not different from other chronic liver diseases. About 15% of all cases with chronic liver diseases show an autoimmune pathogenesis. Therefore, after exclusion of infectious etiology especially by viruses, the determination of different autoantibodies is recommended. PBC is a chronic inflammatory disorder of the small and medium bile ducts and serologically characterized by the occurrence of circulating M2 autoantibodies. M2 autoantibodies belong to the group of mitochondrial antibodies (AMA) and recognize three related proteins of the alpha-keto acid dehydrogenase complex. This complex is located at the inner mitochondrial membrane. The major epitopes of this protein complex is found on the E2 subunit and the protein X of the pyruvate dehydrogenase complex (PDC). Furthermore, M2 autoantibodies bind the E1alpha and E1beta subunits of the same complex and the E2 subunit of several other multi-enzyme complexes, such as the 2-oxo-glutarate dehydrogenase complex (OGDC) and the branched chain 2-oxo acid dehydrogenase complex (BCOADC).M2 autoantibodies have been found in up to 96% of patients suffering from PBC thus representing a powerful serological tool for the diagnosis of PBC.

The Eagle Biosciences Anti-M2 ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of IgG antibodies to the mitochondrial Antigens M2.  The antibodies of the standards, controls, and diluted patient samples react with to the native mitochondrial Antigens M2 immobilized on the solid phase of microtiter plates. The use of highly purified native mitochondrial Antigens M2 guarantees the specific binding of M2 antibodies of the specimen under investigation. Following an incubation period of 30 min at room temperature (RT), unbound serum components are removed by a wash step. The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP) within the incubation period of 30 min at RT. Excessive conjugate is separated from the solid-phase immune complexes by the following wash step. HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethyl¬benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution (HCl) into the wells after 30 min at RT turning the solution from blue to yellow.The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the antibody concentrations of the standards (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve.

ELISA

Assay Kits

Immunology

1 x 96 well

M2

AM231-K01 ASSAY EIA ELISA IMMUNOASSAY KIT, Anti M2 Mitochondrial Antibodies, PBC

3 U/ml

3 -300 U/ml

10 ul

serum, plasma

2 hours

Human

RUO

wet

2-8C