ELISA/assay

ADMA (Asymmetric Dimethylarginine) / Arginine ELISA

AAP31-K02

1 x 96 well

ADMA (Asymmetric Dimethylarginine) / Arginine ELISA

Importantly, high ADMA levels and low L-arginine/ADMA ratio were both independent predictors of death in the community-based Framingham Offspring Study. As ADMA competes with L-arginine for binding to NO synthase, many scientists suggest that the L-arginine/ADMA ratio is a better index of NOS substrate availability and, thus, functional integrity of the NOS pathway, than L-arginine levels alone.

The new competitive ADMA (Asymmetric Dimethylarginine) / Arginine ELISA Kit uses the microtiter plate format. Antigens is bound to the solid phase of the microtiter plate. Antigens in the samples is acylated and competes with solid phase bound Antigens for a fixed number of antiserum binding sites. When the system is in equilibrium, free Antigens and free Antigens-antiserum complexes are removed by washing. The antibody bound to the solid phase ADMA and Arginine, respectively are detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase Antigens is inversely proportional to the Antigens concentration of the sample.

ELISA

Assay Kits

Oxidative Stress

1 x 96 well

ADMA

SDMA, Symmetric Dimethylarginine, ADMA, Arginine, L-Arginine

ADMA: 0.01 umol/L, Arginine: 3.0 umol/L

ADMA: 0.1 - 5.0 umol/L, Arginine: 6 - 600 umol/L

20 uL

EDTA Plasma

Overnight

human

RUO

wet

2-8C