ELISA/assay

Haptogloblin Typing ELISA Assay Kit

710-01

1 x 96 well

690 USD

Haptogloblin Typing ELISA Assay Kit

Haptoglobin (Hp) typing ELISA pates are supplied coated with purified monoclonal antibody (mAb) directed against Hp. The serum to be tested in diluted and incubated in the Hp ELISA plate. In this step Hp in the serum binds to the immobilized antibody. The non-bound Hp is removed by washing. Horseradish Peroxidase (HRP) conjugated monoclonal antibody to Hp is added. Note: the same mAb in the unconjugated form was used to coat the microtiter wells. Since Hp 1-1 is dimeric, at most only one HRP conjugated mAb can bind per dimer attached to the well. However, Hp 2-1 and Hp 2-2 are polymeric and can potentially nind 2-8 HRP conjugated mAbs. Unbound conjugate is removed by washing. TMB substrate reagent is added resulting in development of a blue color. The blue color development is stopped with the addition of stop solution changing the color to yellow.  Absorbance is measured using a spectrophotometer at 450 nm. The absorbance of each sample is compared to a user-calculated cut-off to determine its Hp phenotype.

ELISA

Assay Kits

Endocrinology

1 x 96 well

Haptoglobin

Haptogloblin, Hp, Diabetes

Cut-off

Variable

Diabetic Serum/Plasma

1.5 hours

Human

RUO

wet

2-8C

690 USD