ELISA/assay

RF IgA (Rheumatoid Factor) ELISA

4027

1 x 96 well

455 USD

RF IgA (Rheumatoid Factor) ELISA

The Eagle Biosciences RF IgA ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of IgA antibodies to the Fc region of IgG.The antibodies of the calibrators, control and diluted patient samples react with rabbit IgG immobilized on the solid phase of microtiter plates. Following an incubation period of 60 min at room temperature, unbound sample components are removed by a wash step.The bound IgG antibodies react specifically with anti-human-IgA conjugated to horseradish peroxidase (HRP). Within the incubation period of 30 min at RT, excessive conjugate is separated from the solid-phase immune complexes by the following wash step.HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethyl¬benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at room temperature turning the solution from blue to yellow.The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the antibody concentrations of the calibrators (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve.

ELISA

Assay Kits

Immunology

1 x 96 well

RF

RFA31-K01 ASSAY EIA ELISA IMMUNOASSAY KIT RF Rheumatoid Arthritis, IgA,

1 U/mL

1-300 U/mL

10 ul

serum, plasma

2.5 hours

Human

RUO

wet

2-8C

455 USD