ELISA/assay

IAA (Insulin Autoantibodies) ELISA

3806

1 x 96 well

625 USD

IAA (Insulin Autoantibodies) ELISA

In the first step Insulin AAb from the diluted sample bind to human recombinant insulin coated on the microtiter plate. After an incubation of 60 minutes at 37 °C unbound components are removed by washing. In a next step bound antibodies reacts with the added anti-human-IgG horseradish peroxidase (HRP) complex. Excessive conjugate is removed after 15 minutes at 37 °C by another washing step. HRP converts the colorless substrate TMB added into a blue product. The enzyme reaction is stopped by adding an acid solution after 15 minutes at 37 °C. The absorbance of the resulting yellow product is measured at 450 / 620 nm within 30 minutes. The obtained OD is direct proportional to the amount of bound antibodies.

ELISA

Assay Kits

Endocrinology

1 x 96 well

IAA

IAA, Insulin Autoantibody, antibodies

0.08 U/mL

0.1 - 20 U/mL

5 uL

serum

90 minutes

Human

RUO

wet

2-8C

625 USD