ELISA/assay

SeroCP Quant C. pneumoniae IgA ELISA Assay Kit

293-01

1 x 96 well

520 USD

SeroCP Quant C. pneumoniae IgA ELISA Assay Kit

The Eagle Biosciences SeroCP Quant Plates are supplied coated with purified elementary bodies of C.pneumoniae (TWAR 183) as antigens. The serum to be tested is diluted and incubated in the SeroCP Quant plate for 1h at 37°C. In this step C.pneumoniae antibodies are bound to the immobilized antigens.  Non-specific antibodies are removed by washing.  Anti-human IgA conjugated to Horseradish Peroxidase (HRP) is added and incubated 1h at 37°C. In this step the HRP-Conjugate is bound to the prebound antigen-antibody complex.  Unbound conjugate is removed by washing.  Upon the addition of TMB-Substrate, the Substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced chromogen.  Upon the addition of the Stop Solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620nm.  The absorbance is proportional to the amount of the specific antibodies that are bound to the coated antigens.

ELISA

Assay Kits

Immunology

1 x 96 well

C. pneumoniae IgA

C. pneumoniae IgA, SeroCP, Quant

Cut-off

Cut-off

10uL

Serum

2.25 hours

Human

RUO

wet

2-8C

520 USD