ELISA/assay

SeroCP Quant C. pneumoniae IgG ELISA Assay Kit

291-01

1 x 96 well

520 USD

SeroCP Quant C. pneumoniae IgG ELISA Assay Kit

The Eagle Biosciences SeroCP Quant Plates are supplied coated with purified elementary bodies of C.pneumoniae (TWAR - 183) antigens. The serum to be tested is diluted and incubated in the SeroCP Quant plate for 1h at 37°C. In this step C.pneumoniae antibodies are bound to the immobilized antigens. Non-specific antibodies are removed by washing. Anti-human IgG conjugated to Horseradish Peroxidase (HRP) is added and incubated 1h at 37°C. In this step the HRP-Conjugate is bound to the prebound antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB-Substrate, the Substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced Substrate. Upon the addition of the Stop Solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620nm. The absorbance is proportional to the amount of the specific antibodies that are bound to the coated antigens.

ELISA

Assay Kits

Immunology

1 x 96 well

C. pneumoniae IgG

C. pneumoniae IgG, SeroCP, Quant

Cut-off

Cut-off

10uL

Serum

2.25 hours

Human

RUO

wet

2-8C

520 USD