ELISA/assay

SeroCP C. pneumoniae IgA ELISA Assay Kit

193-01

1 x 96 well

475 USD

SeroCP C. pneumoniae IgA ELISA Assay Kit

The Eagle Biosciences SeroCP Plates are supplied coated with purified elementary bodies of C. pneumoniae (TWAR 183) antigens. * The serum to be tested is diluted and incubated in the SeroCP plate for 1h at 37°C. In this step C.pneumoniae antibodies are bound to the immobilized antigens. * Non-specific antibodies are removed by washing. * Anti-human IgA conjugated to Horseradish Peroxidase (HRP) is added and incubated 1h at 37°C. In this step the HRP-Conjugate is bound to the prebound antigen-antibody complex. * Unbound conjugate is removed by washing. * Upon the addition of TMB-Substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced Substrate. * Upon the addition of the Stop Solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450nm. * The absorbance is proportional to the amount of the specific antibodies that are bound to the coated antigens.

ELISA

Assay Kits

Immunology

1 x 96 well

C. pneumoniae IgA

C. pneumoniae IgA, SeroCP

Cut-off

Cut-off

10uL

Serum

2.25 hours

Human

RUO

wet

2-8C

475 USD