ELISA/assay

SeroCP C. pneumoniae IgM ELISA Assay Kit

192-01

1 x 96 well

500 USD

SeroCP C. pneumoniae IgM ELISA Assay Kit

SeroCP Plates are supplied coated with purified elementary bodies of C. pneumoniae (TWAR 183) antigens. Serum to be tested is diluted and then incubated in the SeroCP plate for 1h at 37°C. In this step C. pneumoniae antibodies are bound to the immobilized antigens. Non-specific antibodies are removed by washing. Anti-human IgM conjugated to Horseradish Peroxidase (HRP) is added and incubated 1h at 37°C. In this step the HRP-Conjugate is bound to the prebound antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB-Substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced substrate. Upon the addition of the Stop Solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450nm. The absorbance is proportional to the amount of the specific antibodies that are bound to the coated antigens.

ELISA

Assay Kits

Immunology

1 x 96 well

C. pneumoniae IgM

C. pneumoniae IgM, SeroCP

Cut-off

Cut-off

5uL

Serum

2.25 hours

Human

RUO

wet

2-8C

500 USD