ELISA/assay

SeroCT C. trachomatis IgA ELISA Assay Kit

183-01

1 x 96 well

475 USD

SeroCT C. trachomatis IgA ELISA Assay Kit

The Eagle Biosciences SeroCT plates are coated with C. trachomatis specific peptides. Serum to be tested is diluted and incubated with the precoated SeroCT plate 1h at 37°C. In this step C. trachomatis specific antibodies are bound to the immobilized C. trachomatis specific peptides. Non specific antibodies are removed by washing. Anti-human IgA conjugated to horseradish peroxidase (HRP) is added and incubated 1h at 37°C. In this step the HRP-conjugate is bound to the prebound antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB-substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced substrate. Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450nm. The absorbance is proportional to the amount of the specific antibodies which are bound to the immobilized peptides.

ELISA

Assay Kits

Immunology

1 x 96 well

C. trachomatis IgA

C. trachomatis IgA, SeroCT

Cut-off

Cut-off

10uL

Serum

2.25 hours

Human

RUO

wet

2-8C

475 USD