ELISA/assay

SeroMP Recombinant M. pneumoniae IgG ELISA Assay Kit

1261-01

1 x 96 well

520 USD

SeroMP Recombinant M. pneumoniae IgG ELISA Assay Kit

SeroMP Recombinant microtiter plates are coated with M. pneumoniae recombinant antigens. The serum to be tested is diluted and incubated in the SeroMP Recombinant plate. In this step, M. pneumoniae specific antibodies are bound to the immobilized antigens.  Non-specific antibodies are removed by washing.  Anti-human IgG conjugated to horseradish peroxidase (HRP) is added. In this step the HRP-conjugate is bound to the prebound antigen-antibody complex.  Unbound conjugate is removed by washing.  Upon the addition of TMB-substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced Substrate.  Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620nm. The absorbance is proportional to the levels of the specific antibodies that are bound to the coated antigens.

ELISA

Assay Kits

Immunology

1 x 96 well

M. pneumoniae IgG

M. pneumoniae IgG, SeroMP

Cut-off

Cut-off

10uL

Serum

2 hours

Human

RUO

wet

2-8C

520 USD