ELISA/assay

SeroCP RT C. pneumoniae IgG ELISA Assay Kit

1191-01

1 x 96 well

520 USD

SeroCP RT C. pneumoniae IgG ELISA Assay Kit

The Eagle Biosciences SeroCP RT Plates are coated with C. pneumoniae specific antigens. Serum to be tested is diluted and incubated with the pre-coated SeroCPTM RT plate 30 minutes at Room Temperature (RT).  In this step C. pneumoniae specific antibodies are bound to the immobilized C. pneumoniae specific antigens. Non-specific antibodies are removed by washing. Anti-human IgG conjugated to horseradish peroxidase (HRP) is added and incubated 30 minutes at Room Temperature.  In this step the HRP-conjugate is bound to the pre-formed antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced substrate. Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620 nm. The absorbance is proportional to the amount of the specific antibodies which are bound to the immobilized C. pneumoniae specific antigens.

ELISA

Assay Kits

Immunology

1 x 96 well

C. pneumoniae IgG

C. pneumoniae IgG, SeroCP RT

Cut-off

Cut-off

10uL

Serum

1.5 hours

Human

RUO

wet

2-8C

520 USD