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easYmer HLA-A*02:01 MHC Tetramers Kit, 500 Tests

1002-01-500

150 Tests

easYmer HLA-A*02:01 MHC Tetramers Kit, 500 Tests

The easYmer MHC Tetramers Kit features a highly active formulation of HLA class I (HLA-I) molecules, which can be used to generate specific peptide-HLA class I monomers of your choice in your own laboratory. These monomers can easily be tetramerized with fluorophore conjugated streptavidin and used to stain antigen specific T-cells for analysis in flow cytometric assays. Optionally, the monomers can be stored frozen for later use. The easYmer technology is highly flexible and suitable for screening of a single epitope in a large number of samples, as well as for screening of a large number of different epitopes in parallel.

This protocol is designed to evaluate the efficiency of peptide-HLA-I interaction and complex formation. The assay is based on detecting the ß2-microglobulin (ß2m? light chain subunit of recombinant HLA Class I (HLA-I) complexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labeled with PE-conjugated anti-human ß2m, and analyzed by flow cytometry. Since peptide-HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub-optimally, or not at all, will give moderate to non-detectable signals.

MHC Tetramers

easYmer

Cancer Biomarkers

150 Tests

RUO

wet

-20C