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easYmer HLA-A*01:01 MHC Tetramers Kit, 150 Tests

1001-01-150

150 Tests

2670 USD

easYmer HLA-A*01:01 MHC Tetramers Kit, 150 Tests

This protocol is designed to evaluate the efficiency of peptide-HLA-I interaction and complex formation. The assay is based on detecting the ß2-microglobulin (ß2m? light chain subunit of recombinant HLA Class I (HLA-I) comlexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labeled with PE-conjugated anti-human ß2m, and analyzed by flow cytometry. Since peptide-HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub-optimally, or not at all, will give moderate to non-detectable signals.

MHC Tetramers

easYmer

Cancer Biomarkers

150 Tests

RUO

wet

-20C

2670 USD