PhosphoDetect™ Anti-Fos (pSer374) Mouse mAb (34E4) | EMD Millipore ST1029-1SET product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PhosphoDetect™ Anti-Fos (pSer374) Mouse mAb (34E4)

catalog :

ST1029-1SET

quantity :

1 set

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, ELISA

product information

Catalog Number :

ST1029-1SET

Category :

Antibodies

Subcategory :

Cell Signaling

Product Name :

PhosphoDetect™ Anti-Fos (pSer374) Mouse mAb (34E4)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

2353 (Hu), 14281 (Ms), 314322 (Rt)

Host Name :

Clone :

34E4

Conjugate :

Purified

Isotype :

IgG1

Product Description :

PhosphoDetect™ Anti-Fos (pSer374) Mouse mAb (34E4)

Cross Reactivity :

Human

Background :

The early gene product c-Fos is expressed following mitogenic stimulation and functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at Ser374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at Thr325.

ALT Names :

AP-1, C-FOS, D12Rfj1, V-FOS, FBJ murine osteosarcoma viral oncogene homolog

Immunogen :

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 374 phosphorylation site of human c-Fos

Specificity :

Recognizes the ~55 kDa c-fos protein phosphorylated at Ser 374 by MAP kinase.

Package Size :

1 set

Uses :

ELISA, WB

Storage :

Reconstitute antibody with 1 ml water (15 minutes, room temperature). Following reconstitution, aliquot and freeze in liquid nitrogen. Reconstituted antibody can be stored at -80°C for up to 1 year. Aliquots may be stored at 4°C for up to 3 months and should be thawed at 37°C. Reconstitute control lysate with 200 ul H 2 O. After complete solubilization of the proteins add 200 ul SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconsitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles. Avoid repeated freeze-thaw.