PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9) | EMD Millipore PS1023-50UL product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9)

catalog :

PS1023-50UL

quantity :

50 uL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

product information

Catalog Number :

PS1023-50UL

Category :

Antibodies

Subcategory :

Epigenetics & Nuclear Function

Product Name :

PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

4088 (Hu), 17127 (Ms), 25631 (Rt)

Host Name :

Clone :

C25A9

Conjugate :

Purified

Isotype :

IgG

Product Description :

PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9)

Cross Reactivity :

Human

Background :

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-beta signals from the cell surface to the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.Following stimulation by TGF-beta, Smad2 and Smad3 become phosphorylated at carboxyl terminal serine residues (Ser465/467 on Smad2; Ser423/425 on Smad3) by TGF-beta Receptor I.

ALT Names :

AU022421, DKFZP586N0721, DKFZp686J10186, hMAD-3, HSPC193, HsT17436, JV15-2, MAD3, MADH3, MGC60396, SMAD family member 3

Immunogen :

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 423/425 phosphorylation sites of Smad3, coupled to KLH.

Specificity :

Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser 423/425 .

Package Size :

50 uL

Uses :

WB, IP

Storage :

Do not aliquot.