mTOR/FRAP (Ab-2) Mouse mAb (22C2) | EMD Millipore OP97-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

mTOR/FRAP (Ab-2) Mouse mAb (22C2)

catalog :

OP97-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 2	Felzen V, Hiebel C, Koziollek Drechsler I, ReiÃŸig S, Wolfrum U, KÃ¶gel D, et al. Estrogen receptor Î± regulates non-canonical autophagy that provides stress resistance to neuroblastoma and breast cancer cells and involves BAG3 function. Cell Death Dis. 2015;6:e1812 pubmed publisher

product information

Catalog Number :

OP97-100UG

Category :

Antibodies

Subcategory :

Cell Signaling

Product Name :

Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

2475 (Hu), 56717 (Ms), 56718 (Rt)

Host Name :

Clone :

22C2

Isotype :

IgG1

Product Description :

Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2)

Cross Reactivity :

Human

Background :

TOR1 is one of three genes (FPR1, TOR1, and TOR2) mutations of which confer rapamycin resistance in the yeast Saccharomyces cerevisiae. The proteins encoded by the TOR1 and TOR2 genes are large (250 kDa) in size, have homology to the phosphatidylinositol and protein kinases, and TOR2 possesses PI 4-kinase activity. The TOR proteins and their mammalian homolog (FRAP, mTOR) form a complex with a 12 kDa cytosolic protein, FKBP-12 and rapamycin to effect arrest in the G1 phase of the cell cycle. The G1 block is mediated by the activation of ribosomal p70S6K and cyclin dependent kinases. The cell cycle arrest activity requires the presence of the intact kinase domain in the TOR protein and in particular a conserved Ser residue within the domain. The human hTOR (mammalian or mTOR) protein shows 39-43% homology with the S. cerevisiae proteins TOR1 and TOR2. Ser2448 has been identified as a PKB-mediated phosphorylation site.

ALT Names :

Flat-Top, FLJ44809, FRAP, FRAP1, FRAP2, FRB, MGC118056, RAFT1, RAPT1, RRAFT1, mechanistic target of rapamycin (serine/threonine kinase)

Immunogen :

a synthetic peptide corresponding to amino acids 230-240 of human TOR

Specificity :

Recognizes the ~290 kDa mTOR/FRAP protein.

Package Size :

100 ug

Uses :

WB, IP

Storage :

Do not freeze