BRCA2 (Ab-1) Mouse mAb (2B) | EMD Millipore OP95-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

BRCA2 (Ab-1) Mouse mAb (2B)

catalog :

OP95-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:10,000; loading ...; fig s11d	Bahal R, Ali McNeer N, Quijano E, Liu Y, Sulkowski P, Turchick A, et al. In vivo correction of anaemia in ?-thalassemic mice by ?PNA-mediated gene editing with nanoparticle delivery. Nat Commun. 2016;7:13304 pubmed publisher
western blot; human; fig 1	Michl J, Zimmer J, Buffa F, McDermott U, Tarsounas M. FANCD2 limits replication stress and genome instability in cells lacking BRCA2. Nat Struct Mol Biol. 2016;23:755-757 pubmed publisher
western blot; human; fig 7h	Choe K, Nicolae C, Constantin D, Imamura Kawasawa Y, Delgado Diaz M, De S, et al. HUWE1 interacts with PCNA to alleviate replication stress. EMBO Rep. 2016;17:874-86 pubmed publisher
western blot; human; fig 2	De Toni E, Ziesch A, Rizzani A, TÃ¶rÃ¶k H, Hocke S, LÃ¼ S, et al. Inactivation of BRCA2 in human cancer cells identifies a subset of tumors with enhanced sensitivity towards death receptor-mediated apoptosis. Oncotarget. 2016;7:9477-90 pubmed publisher
western blot; human; fig 1	Hasvold G, Lund Andersen C, Lando M, Patzke S, Hauge S, Suo Z, et al. Hypoxia-induced alterations of G2 checkpoint regulators. Mol Oncol. 2016;10:764-73 pubmed publisher
western blot; human; fig s11	Castella M, Jacquemont C, Thompson E, Yeo J, Cheung R, Huang J, et al. FANCI Regulates Recruitment of the FA Core Complex at Sites of DNA Damage Independently of FANCD2. PLoS Genet. 2015;11:e1005563 pubmed publisher
immunocytochemistry; human; fig 3	Sowd G, Mody D, Eggold J, Cortez D, Friedman K, Fanning E. SV40 utilizes ATM kinase activity to prevent non-homologous end joining of broken viral DNA replication products. PLoS Pathog. 2014;10:e1004536 pubmed publisher
	Zhao W, Steinfeld J, Liang F, Chen X, Maranon D, Jian Ma C, et al. BRCA1-BARD1 promotes RAD51-mediated homologous DNA pairing. Nature. 2017;550:360-365 pubmed publisher
	von Nicolai C, Ehlén A, Martin C, Zhang X, Carreira A. A second DNA binding site in human BRCA2 promotes homologous recombination. Nat Commun. 2016;7:12813 pubmed publisher

product information

Catalog Number :

OP95-100UG

Category :

Antibodies

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-BRCA2 (Ab-1) Mouse mAb (2B)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

675 (Hu), 12190 (Ms), 360254 (Rt)

Host Name :

Clone :

Conjugate :

Purified

Isotype :

IgG2b

Product Description :

Anti-BRCA2 (Ab-1) Mouse mAb (2B)

Cross Reactivity :

Human

Background :

Inherited mutations in the BRCA2 gene enhances the risk of familial breast cancer with the neoplasia related to loss of the wild type allele. Human BRCA2 encodes a 3418 amino acid protein with little homology to any known proteins. Transcription of BRCA2 appears to be co-ordinately regulated with BRCA1 during proliferation and differentiation with low level expression in early G1 and maximal expression occurring during the G1/S transition of the cell cycle. While loss of BRCA2 is associated with early onset breast cancer, the gene is also critical for embryogenesis. Although some mice nullizygous for BRCA2 die during embryogenesis, there have been BRCA2 knock-out mice that do survive to adulthood, albeit with various defects particularly in DNA repair capability. Loss of BRCA2 expression in embryogenesis does not appear to affect apoptosis, per se, but proliferation is impaired, perhaps due to enhanced expression of p21WAF1.

ALT Names :

AI256696, AW045498, BRCC2, BROVCA2, FACD, FAD, FAD1, FANCB, FANCD, FANCD1, RAB163, breast cancer 2, early onset

Immunogen :

a recombinant protein consisting of amino acids 1651-1821 of human BRCA2 fused to GST

Specificity :

Recognizes the ~460 kDa BRCA2 protein.

Package Size :

100 ug

Uses :

WB, IP

Storage :

Following initial thaw, aliquot and freeze (-20°C).