p21WAF1 (58-77) (Ab-1) Mouse mAb (EA10) Fluorescein Conjugate | EMD Millipore OP64F-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

p21WAF1 (58-77) (Ab-1) Mouse mAb (EA10) Fluorescein Conjugate

catalog :

OP64F-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, flow cytometry

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 1c	Miyamoto T, Lo P, Saichi N, Ueda K, Hirata M, Tanikawa C, et al. Argininosuccinate synthase 1 is an intrinsic Akt repressor transactivated by p53. Sci Adv. 2017;3:e1603204 pubmed publisher
western blot; human; 1:1000; fig 1	Eichner R, Heider M, Fernández Sáiz V, van Bebber F, Garz A, Lemeer S, et al. Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity. Nat Med. 2016;22:735-43 pubmed publisher
western blot; human; 1:200; fig 6	Marthandan S, Baumgart M, Priebe S, Groth M, Schaer J, Kaether C, et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS ONE. 2016;11:e0154531 pubmed publisher
western blot; human; fig 2	Beard J, Tenga A, Hills J, Hoyer J, Cherian M, Wang Y, et al. The orphan nuclear receptor NR4A2 is part of a p53-microRNA-34 network. Sci Rep. 2016;6:25108 pubmed publisher
western blot; human; loading ...; fig 1c	Koguchi T, Tanikawa C, Mori J, Kojima Y, Matsuda K. Regulation of myo-inositol biosynthesis by p53-ISYNA1 pathway. Int J Oncol. 2016;48:2415-24 pubmed publisher
western blot; human; 1:1000; fig 4	Ercilla A, Llopis A, Feu S, Aranda S, Ernfors P, Freire R, et al. New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress. Nucleic Acids Res. 2016;44:4745-62 pubmed publisher
western blot; human; fig 4	Wang Y, Xu Q, Sack L, Kang C, Elledge S. A gain-of-function senescence bypass screen identifies the homeobox transcription factor DLX2 as a regulator of ATM-p53 signaling. Genes Dev. 2016;30:293-306 pubmed publisher
western blot; human; 1:1000; fig 8	DomÃnguez D, Feijoo P, Bernal A, Ercilla A, Agell N, GenescÃ A, et al. Centrosome aberrations in human mammary epithelial cells driven by cooperative interactions between p16INK4a deficiency and telomere-dependent genotoxic stress. Oncotarget. 2015;6:28238-56 pubmed publisher
western blot; human	Kim J, Sato M, Choi J, Kim H, Yeh B, Larsen J, et al. Nuclear Receptor Expression and Function in Human Lung Cancer Pathogenesis. PLoS ONE. 2015;10:e0134842 pubmed publisher
western blot; human; 1:200; fig 1	Chen L, Rousseau R, Middleton S, Nichols G, Newell D, Lunec J, et al. Pre-clinical evaluation of the MDM2-p53 antagonist RG7388 alone and in combination with chemotherapy in neuroblastoma. Oncotarget. 2015;6:10207-21 pubmed
western blot; human; fig 1	Toropainen S, Malinen M, Kaikkonen S, Rytinki M, JÃ¤Ã¤skelÃ¤inen T, Sahu B, et al. SUMO ligase PIAS1 functions as a target gene selective androgen receptor coregulator on prostate cancer cell chromatin. Nucleic Acids Res. 2015;43:848-61 pubmed publisher

product information

Catalog Number :

OP64F-100UG

Category :

Antibodies

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-p21WAF1 (58-77) (Ab-1) Mouse mAb (EA10) Fluorescein Conjugate

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

1026 (Hu), 12575 (Ms), 114851 (Rt)

Host Name :

Clone :

EA10

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-p21WAF1 (58-77) (Ab-1) Mouse mAb (EA10) Fluorescein Conjugate

Cross Reactivity :

Human

Background :

The tumor suppressor p53 transcriptionally activates a number of genes including the WAF1/CIP1 gene in response to DNA damage. The 21 kDa product of the WAF1 gene is found in a complex involving cyclins, cyclin dependent kinases (CDK), and PCNA in normal cells but not transformed cells and appears to be a universal inhibitor of CDK activity. One consequence of p21WAF1 binding to and inhibiting CDKs is to prevent CDK-dependent phosphorylation and subsequent inactivation of the Rb protein which is essential for cell cycle progression. p21WAF1 is, therefore, a potent and reversible inhibitor of cell cycle progression at both the G1 and G2 checkpoints, presumably to allow sufficient time for DNA repair to be completed. Irreversible G1 or G2 arrest leads to apoptosis. However, the role of p21WAF1 in apoptosis is less clear although p53-mediated apoptosis leads to increased WAF1 expression.

ALT Names :

CAP20, CDKI, CDKN1, CDKNA1, CIP1, MDA-6, P21, p21/WAF1, P21CIP1, P21WAF, p21waf1, SDI1, WAF1, cyclin-dependent kinase inhibitor 1A (p21, Cip1)

Immunogen :

full-length, recombinant, human p21 WAF1

Specificity :

Recognizes the ~21 kDa p21 WAF1 protein.

Package Size :

100 ug

Uses :

FC, IF

Storage :

Following initial thaw, aliquot and freeze (-20°C). Avoid repeated freeze-thaw. Protect from light.