p21WAF1 (Ab-1) Mouse mAb (EA10) | EMD Millipore OP64-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

p21WAF1 (Ab-1) Mouse mAb (EA10)

catalog :

OP64-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

citations: 19

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:500; loading ...; fig 9b	Lang A, Whongsiri P, Yilmaz M, Lautwein T, Petzsch P, Greife A, et al. Knockdown of UTX/KDM6A Enriches Precursor Cell Populations in Urothelial Cell Cultures and Cell Lines. Cancers (Basel). 2020;12: pubmed publisher
western blot; human; fig 1c	Miyamoto T, Lo P, Saichi N, Ueda K, Hirata M, Tanikawa C, et al. Argininosuccinate synthase 1 is an intrinsic Akt repressor transactivated by p53. Sci Adv. 2017;3:e1603204 pubmed publisher
western blot; human; fig 5a	Coaxum S, Tiedeken J, Garrett Mayer E, Myers J, Rosenzweig S, Neskey D. The tumor suppressor capability of p53 is dependent on non-muscle myosin IIA function in head and neck cancer. Oncotarget. 2017;8:22991-23007 pubmed publisher
western blot; human; fig 3a	Mori J, Tanikawa C, Ohnishi N, Funauchi Y, Toyoshima O, Ueda K, et al. EPSIN 3, A Novel p53 Target, Regulates the Apoptotic Pathway and Gastric Carcinogenesis. Neoplasia. 2017;19:185-195 pubmed publisher
western blot; human; 1:1000; fig 1	Eichner R, Heider M, Fernández Sáiz V, van Bebber F, Garz A, Lemeer S, et al. Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity. Nat Med. 2016;22:735-43 pubmed publisher
western blot; human; 1:200; fig 6	Marthandan S, Baumgart M, Priebe S, Groth M, Schaer J, Kaether C, et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS ONE. 2016;11:e0154531 pubmed publisher
western blot; human; fig 2	Beard J, Tenga A, Hills J, Hoyer J, Cherian M, Wang Y, et al. The orphan nuclear receptor NR4A2 is part of a p53-microRNA-34 network. Sci Rep. 2016;6:25108 pubmed publisher
western blot; human; loading ...; fig 1c	Koguchi T, Tanikawa C, Mori J, Kojima Y, Matsuda K. Regulation of myo-inositol biosynthesis by p53-ISYNA1 pathway. Int J Oncol. 2016;48:2415-24 pubmed publisher
western blot; human; 1:1000; fig 4	Ercilla A, Llopis A, Feu S, Aranda S, Ernfors P, Freire R, et al. New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress. Nucleic Acids Res. 2016;44:4745-62 pubmed publisher
western blot; human; fig 4	Wang Y, Xu Q, Sack L, Kang C, Elledge S. A gain-of-function senescence bypass screen identifies the homeobox transcription factor DLX2 as a regulator of ATM-p53 signaling. Genes Dev. 2016;30:293-306 pubmed publisher
western blot; human; 1:1000; fig 8	DomÃnguez D, Feijoo P, Bernal A, Ercilla A, Agell N, GenescÃ A, et al. Centrosome aberrations in human mammary epithelial cells driven by cooperative interactions between p16INK4a deficiency and telomere-dependent genotoxic stress. Oncotarget. 2015;6:28238-56 pubmed publisher
western blot; human	Kim J, Sato M, Choi J, Kim H, Yeh B, Larsen J, et al. Nuclear Receptor Expression and Function in Human Lung Cancer Pathogenesis. PLoS ONE. 2015;10:e0134842 pubmed publisher
western blot; human; 1:200; fig 1	Chen L, Rousseau R, Middleton S, Nichols G, Newell D, Lunec J, et al. Pre-clinical evaluation of the MDM2-p53 antagonist RG7388 alone and in combination with chemotherapy in neuroblastoma. Oncotarget. 2015;6:10207-21 pubmed
western blot; human; fig 1	Toropainen S, Malinen M, Kaikkonen S, Rytinki M, JÃ¤Ã¤skelÃ¤inen T, Sahu B, et al. SUMO ligase PIAS1 functions as a target gene selective androgen receptor coregulator on prostate cancer cell chromatin. Nucleic Acids Res. 2015;43:848-61 pubmed publisher
immunohistochemistry; human; 1:100; tbl 1	Sevcenco S, Haitel A, Ponhold L, Susani M, Fajkovic H, Shariat S, et al. Quantitative apparent diffusion coefficient measurements obtained by 3-Tesla MRI are correlated with biomarkers of bladder cancer proliferative activity. PLoS ONE. 2014;9:e106866 pubmed publisher
	Kline C, van den Heuvel A, Allen J, Prabhu V, Dicker D, El Deiry W. ONC201 kills solid tumor cells by triggering an integrated stress response dependent on ATF4 activation by specific eIF2Î± kinases. Sci Signal. 2016;9:ra18 pubmed publisher
	Matsuda Y, Schlange T, Oakeley E, Boulay A, Hynes N. WNT signaling enhances breast cancer cell motility and blockade of the WNT pathway by sFRP1 suppresses MDA-MB-231 xenograft growth. Breast Cancer Res. 2009;11:R32 pubmed publisher
	Kalemi T, Papazisis K, Lambropoulos A, Voyatzi S, Kotsis A, Kortsaris A. Expression of the HER family mRNA in breast cancer tissue and association with cell cycle inhibitors p21(waf1) and p27(kip1). Anticancer Res. 2007;27:913-20 pubmed
	Fraser M, Watson P, Fraig M, Kelley J, Nelson P, Boylan A, et al. CaSm-mediated cellular transformation is associated with altered gene expression and messenger RNA stability. Cancer Res. 2005;65:6228-36 pubmed

product information

Catalog Number :

OP64-100UG

Category :

Antibodies

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-p21WAF1 (Ab-1) Mouse mAb (EA10)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

1026 (Hu), 12575 (Ms), 114851 (Rt)

Host Name :

Clone :

EA10

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-p21WAF1 (Ab-1) Mouse mAb (EA10)

Cross Reactivity :

Human

Background :

The tumor suppressor p53 transcriptionally activates a number of genes including the WAF1/CIP1 gene in response to DNA damage. The ~21 kDa product of the WAF1 gene is found in a complex involving cyclins, cyclin dependent kinases (CDK), and PCNA in normal cells but not transformed cells and appears to be a universal inhibitor of CDK activity. One consequence of p21WAF1 binding to and inhibiting CDKs is to prevent CDK-dependent phosphorylation and subsequent inactivation of the Rb protein which is essential for cell cycle progression. p21WAF1 is, therefore, a potent and reversible inhibitor of cell cycle progression at both the G1 and G2 checkpoints, presumably to allow sufficient time for DNA repair to be completed. Irreversible G1 or G2 arrest leads to apoptosis. However, the role of p21WAF1 in apoptosis is less clear although p53-mediated apoptosis leads to increased WAF1 expression.

ALT Names :

CAP20, CDKI, CDKN1, CDKNA1, CIP1, MDA-6, P21, p21/WAF1, P21CIP1, P21WAF, p21waf1, SDI1, WAF1, cyclin-dependent kinase inhibitor 1A (p21, Cip1)

Immunogen :

full-length, recombinant, human p21 WAF1

Specificity :

Recognizes the ~21 kDa p21 WAF1 protein.

Package Size :

100 ug

Uses :

FC, IH, WB, IF, IP, IH(P)

Storage :

Do not freeze