MDM2 (Ab-1) Mouse mAb (IF2) | EMD Millipore OP46-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MDM2 (Ab-1) Mouse mAb (IF2)

catalog :

OP46-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1a	Kayama K, Watanabe S, Takafuji T, Tsuji T, Hironaka K, Matsumoto M, et al. GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis. EMBO Rep. 2017;18:123-137 pubmed publisher
western blot; human; loading ...; fig 5f	Horn T, Ferretti S, Ebel N, Tam A, Ho S, Harbinski F, et al. High-Order Drug Combinations Are Required to Effectively Kill Colorectal Cancer Cells. Cancer Res. 2016;76:6950-6963 pubmed
western blot; mouse; loading ...; fig 1h	Bi P, Yue F, Karki A, Castro B, Wirbisky S, Wang C, et al. Notch activation drives adipocyte dedifferentiation and tumorigenic transformation in mice. J Exp Med. 2016;213:2019-37 pubmed publisher
western blot; human; 1:300; loading ...; fig 3d	Esfandiari A, Hawthorne T, Nakjang S, Lunec J. Chemical Inhibition of Wild-Type p53-Induced Phosphatase 1 (WIP1/PPM1D) by GSK2830371 Potentiates the Sensitivity to MDM2 Inhibitors in a p53-Dependent Manner. Mol Cancer Ther. 2016;15:379-91 pubmed publisher
western blot; human; 1:200; fig s1	Chen L, Rousseau R, Middleton S, Nichols G, Newell D, Lunec J, et al. Pre-clinical evaluation of the MDM2-p53 antagonist RG7388 alone and in combination with chemotherapy in neuroblastoma. Oncotarget. 2015;6:10207-21 pubmed
western blot; human; fig 1	Iannetti A, Ledoux A, Tudhope S, Sellier H, Zhao B, Mowla S, et al. Regulation of p53 and Rb links the alternative NF-ÎºB pathway to EZH2 expression and cell senescence. PLoS Genet. 2014;10:e1004642 pubmed publisher

product information

Catalog Number :

OP46-100UG

Category :

Antibodies

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-MDM2 (Ab-1) Mouse mAb (IF2)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

4193 (Hu), 17246 (Ms), 314856 (Rt)

Host Name :

Clone :

IF2

Conjugate :

Purified

Isotype :

IgG2b

Product Description :

Anti-MDM2 (Ab-1) Mouse mAb (IF2)

Cross Reactivity :

Human

Background :

Increased expression of proto-oncogenes due to gene amplification can lead to cellular transformation in the absence of other mutagenic events. Recently, a novel gene termed MDM2, first identified in mouse as a small acentromeric extrachromosomal element, was shown to become oncogenic upon amplification and tumorigenic when overexpressed in NIH3T3 and Rat2 cells. The human homolog of MDM2 gene has been isolated and mapped to chromosome 12q13-q14, a region that is found to be amplified in human sarcomas. Sequence analysis of the murine, rat, and human MDM2 genes suggests that MDM2 may be involved in transcriptional regulation. MDM2 contains two putative metal-binding motifs, one of which shares similarity to known zinc finger transcriptional activators. MDM2 also contains a putative nuclear localization signal and an acidic domain of the type found in transcriptional activators.

ALT Names :

HDM2, HDMX, MDM2-A1, MGC5370, MGC71221, Transformed mouse 3t3 cell double minute 2, Mdm2 p53 binding protein homolog (mouse)

Immunogen :

human MDM2

Specificity :

Recognizes the ~90 kDa (apparent MW) MDM2 protein. Also recognizes isoforms at ~57 and ~74/76 kDa.

Package Size :

100 ug

Uses :

IH, WB, IF, IP, IH(P)

Storage :

Do not freeze