p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) | EMD Millipore OP43L-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

catalog :

OP43L-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:100; fig 3	Meitinger F, Anzola J, Kaulich M, Richardson A, Stender J, Benner C, et al. 53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration. J Cell Biol. 2016;214:155-66 pubmed publisher
western blot; human; fig 5i	Capell B, Drake A, Zhu J, Shah P, Dou Z, Dorsey J, et al. MLL1 is essential for the senescence-associated secretory phenotype. Genes Dev. 2016;30:321-36 pubmed publisher
western blot; human; fig 4	Funauchi Y, Tanikawa C, Yi Lo P, Mori J, Daigo Y, Takano A, et al. Regulation of iron homeostasis by the p53-ISCU pathway. Sci Rep. 2015;5:16497 pubmed publisher

product information

Catalog Number :

OP43L-100UG

Category :

Antibodies

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

7157 (Hu), 22059 (Ms), 24842 (Rt)

Host Name :

Clone :

DO-1

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

Cross Reactivity :

Feline, Human

Background :

The human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1, and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death.

ALT Names :

bbl, bfy, bhy, Delta N p53, FLJ92943, LFS1, MGC112612, P53, Trp53, tumor protein p53

Immunogen :

wild type, recombinant, human p53

Specificity :

Recognizes the ~53 kDa mutant and wild-type forms of p53.

Package Size :

100 ug

Uses :

IH, EMSA, WB, IC, IP, IH(P)

Storage :

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 ug/ml. Reconstituted product will be more stable if 0.1% sodium azide is added (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two hours. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.