c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5) | EMD Millipore OP15L-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5)

catalog :

OP15L-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 3	Kim M, Caplen N, Chakka S, Hernandez L, House C, Pongas G, et al. Identification of therapeutic targets applicable to clinical strategies in ovarian cancer. BMC Cancer. 2016;16:678 pubmed publisher
western blot; human; 1:1000; fig 2	Gao S, Chen X, Jin H, Ren S, Liu Z, Fang X, et al. Overexpression of ErbB2 renders breast cancer cells susceptible to 3-BrPA through the increased dissociation of hexokinase II from mitochondrial outer membrane. Oncol Lett. 2016;11:1567-1573 pubmed

product information

Catalog Number :

OP15L-100UG

Category :

Antibodies

Subcategory :

Cell Signaling

Product Name :

Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

2064 (Hu), 13866 (Ms), 24337 (Rt)

Host Name :

Clone :

3B5

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5)

Cross Reactivity :

Human, Mouse

Background :

Amplification of the human proto-oncogene c-neu has been reported to occur in approximately 30% of breast carcinomas and measurement of the amplification has been proposed as a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded p185c-neu is thought to transform cells by chronically stimulating signal transduction pathways, and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal Growth Factor Receptor) and was the second of four members of the EGF receptor family to be described.

ALT Names :

C-ERBB2, c-neu, CD340, EGFR2, ERBB2 RECEPTOR, HER-2, HER2/NEU, mKIAA3023, NEU, NGL, p185erbb2, p185HER2, P185NEU, TKR1, V-ERBB2 NEURO/GLIOBLASTOMA-DERIVED ONCOGENE, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3

Immunogen :

a synthetic peptide (TAENPEYLGLDVPV) corresponding to amino acids 1242-1255 from the C-terminal domain of human c-ErbB2/c-Neu

Specificity :

Recognizes the ~185 kDa c-ErbB2/c-Neu protein.

Package Size :

100 ug

Uses :

IH, WB, IF, IP, IH(P)

Storage :

Reconstitue the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 ug/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long term storage of reconstituted product; repetitive freezing and thawing should be avoided.