MDM2 (Ab-3) Mouse mAb (4B11) | EMD Millipore OP143-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MDM2 (Ab-3) Mouse mAb (4B11)

catalog :

OP143-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1c	Liu T, Xiong J, Yi S, Zhang H, Zhou S, Gu L, et al. FKBP12 enhances sensitivity to chemotherapy-induced cancer cell apoptosis by inhibiting MDM2. Oncogene. 2017;36:1678-1686 pubmed publisher
immunocytochemistry; human; 1:25; fig s2	Swetzig W, Wang J, Das G. Estrogen receptor alpha (ERÎ±/ESR1) mediates the p53-independent overexpression of MDM4/MDMX and MDM2 in human breast cancer. Oncotarget. 2016;7:16049-69 pubmed publisher
western blot; human; fig 3	Tonsing Carter E, Bailey B, Saadatzadeh M, Ding J, Wang H, Sinn A, et al. Potentiation of Carboplatin-Mediated DNA Damage by the Mdm2 Modulator Nutlin-3a in a Humanized Orthotopic Breast-to-Lung Metastatic Model. Mol Cancer Ther. 2015;14:2850-63 pubmed publisher

product information

Catalog Number :

OP143-100UG

Category :

Antibodies

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-MDM2 (Ab-3) Mouse mAb (4B11)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

4193 (Hu), 17246 (Ms), 314856 (Rt)

Host Name :

Clone :

4B11

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-MDM2 (Ab-3) Mouse mAb (4B11)

Cross Reactivity :

Human, Mouse

Background :

Increased expression of proto-oncogenes due to gene amplification can lead to cellular transformation in the absence of other mutagenic events. Recently, a novel gene termed MDM2, first identified in mouse as a small acentromeric extra-chromosomal element, was shown to become oncogenic upon amplification and tumorigenic when overexpressed in NIH/3T3 and Rat2 cells. The human homolog of MDM2 gene has been isolated and mapped to chromosome 12q13-q14, a region that is found to be amplified in human sarcomas. Sequence analysis of the murine, rat, and human MDM2 genes suggests that MDM2 may be involved in transcriptional regulation. MDM2 contains two putative metal-binding motifs, one of which shares similarity to known zinc finger transcriptional activators. MDM2 also contains a putative nuclear localization signal and an acidic domain of the type found in transcriptional activators.

ALT Names :

HDM2, HDMX, MDM2-A1, MGC5370, MGC71221, Transformed mouse 3t3 cell double minute 2, Mdm2 p53 binding protein homolog (mouse)

Immunogen :

full-length, recombinant human MDM2

Specificity :

Recognizes the ~90 kDa (apparent MW) MDM2 protein.

Package Size :

100 ug

Uses :

WB, IF, IP, IH(P)

Storage :

Following initial thaw, aliquot and freeze (-20°C). Avoid repeated freeze-thaw.