Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32) | EMD Millipore NE1023-100UL product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32)

catalog :

NE1023-100UL

quantity :

100 uL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:1000; loading ...	Nagahara Y, Shimazawa M, Ohuchi K, Ito J, Takahashi H, Tsuruma K, et al. GPNMB ameliorates mutant TDP-43-induced motor neuron cell death. J Neurosci Res. 2017;95:1647-1665 pubmed publisher
immunohistochemistry; rat; 1:1000; loading ...; fig 2b	Biró L, Toth M, Sipos E, Bruzsik B, Tulogdi A, Bendahan S, et al. Structural and functional alterations in the prefrontal cortex after post-weaning social isolation: relationship with species-typical and deviant aggression. Brain Struct Funct. 2017;222:1861-1875 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig s1a	Sanges D, Simonte G, Di Vicino U, Romo N, Pinilla I, Nicolas M, et al. Reprogramming Müller glia via in vivo cell fusion regenerates murine photoreceptors. J Clin Invest. 2016;126:3104-16 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 4f	Anastasiadou S, KnÃ¶ll B. The multiple sclerosis drug fingolimod (FTY720) stimulates neuronal gene expression, axonal growth and regeneration. Exp Neurol. 2016;279:243-260 pubmed publisher
immunocytochemistry; mouse; 1:5000; fig 7 immunocytochemistry; rat; 1:5000	Haines J, Herbin O, de la Hera B, Vidaurre O, Moy G, Sun Q, et al. Nuclear export inhibitors avert progression in preclinical models of inflammatory demyelination. Nat Neurosci. 2015;18:511-20 pubmed publisher
	Lin M, Liao P, Chen H, Chang C, Chen S, Chern Y. Degeneration of ipRGCs in Mouse Models of Huntington's Disease Disrupts Non-Image-Forming Behaviors Before Motor Impairment. J Neurosci. 2019;39:1505-1524 pubmed publisher

product information

Catalog Number :

NE1023-100UL

Category :

Antibodies

Subcategory :

Neuroscience

Product Name :

Anti-Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

10460 (Hu), 21335 (Ms), 360962 (Rt)

Host Name :

Clone :

SMI-32

Conjugate :

Unpurified

Isotype :

IgG1

Product Description :

Anti-Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32)

Cross Reactivity :

Mammalian

Background :

Neurofilaments are a type of intermediate filament that serve as major constituent of the cytoskeleton of axons. They are the most abundant fibrillar components of the axon and are composed of three intertwined protofibrils. The neurofilament triplet proteins (NF-L, 68/70 kDa; NF-M, 160 kDa; and NF-H, 200 kDa) are found in both the central and peripheral nervous system and are usually neuron-specific. NF-H and NF-M both require the presence of NF-L protein to co-assemble. NF-H and NF-M become highly phosphorylated after newly formed neurofilaments enter the axon. Neurofilament staining is observed in neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas, and have also been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung are also known to express neurofilaments.

ALT Names :

Aint, C86661, ERIC1, Maskin, MGC117382, MGC133242, transforming, acidic coiled-coil containing protein 3

Immunogen :

homogenized hypothalami from Fischer 344 rat brain

Specificity :

Recognizes the ~180-200 kDa non-phosphorylated neurofilament H protein.

Package Size :

100 uL

Uses :

ELISA, IH, WB, IC, IH(P)

Storage :

Following initial thaw, aliquot and freeze (-20°C). Avoid repeated freeze-thaw.