Replication Protein A (Ab-3) Mouse mAb (RPA34-20) | EMD Millipore NA19L-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Replication Protein A (Ab-3) Mouse mAb (RPA34-20)

catalog :

NA19L-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, dot blot

citations: 5

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; loading ...; fig 4d	Feng X, Hsu S, Kasbek C, Chaiken M, Price C. CTC1-mediated C-strand fill-in is an essential step in telomere length maintenance. Nucleic Acids Res. 2017;45:4281-4293 pubmed publisher
immunocytochemistry; human; 1:100; loading ...; fig st1 western blot; human; 1:1000; loading ...; fig st1	Xu H, Di Antonio M, McKinney S, Mathew V, Ho B, O Neil N, et al. CX-5461 is a DNA G-quadruplex stabilizer with selective lethality in BRCA1/2 deficient tumours. Nat Commun. 2017;8:14432 pubmed publisher
dot blot; human; 1:500; loading ...; fig 2a	Li C, Miao Y, Lingeman R, Hickey R, Malkas L. Partial Purification of a Megadalton DNA Replication Complex by Free Flow Electrophoresis. PLoS ONE. 2016;11:e0169259 pubmed publisher
western blot; human; 1:5000; loading ...; fig 4e	Despras E, Sittewelle M, Pouvelle C, Delrieu N, Cordonnier A, Kannouche P. Rad18-dependent SUMOylation of human specialized DNA polymerase eta is required to prevent under-replicated DNA. Nat Commun. 2016;7:13326 pubmed publisher
immunocytochemistry; mouse; 1:100; fig 1	Ahuja A, Jodkowska K, Teloni F, Bizard A, Zellweger R, Herrador R, et al. A short G1 phase imposes constitutive replication stress and fork remodelling in mouse embryonic stem cells. Nat Commun. 2016;7:10660 pubmed publisher

product information

Catalog Number :

NA19L-100UG

Category :

Antibodies

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Replication Protein A (Ab-3) Mouse mAb (RPA34-20)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

6117 (Hu), 68275 (Ms), 287524 (Rt)

Host Name :

Clone :

RPA34-20

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Replication Protein A (Ab-3) Mouse mAb (RPA34-20)

Cross Reactivity :

Human, Mouse, Yeast

Background :

The replication system of a cell contains several essential components among which are replication factor A (RFA, RPA, or HeLa cell SSB), PCNA, and replication factor C (RFC) as well as several other proteins. RPA, a multi-subunit protein containing a 70 kDa, 34 kDa, and 11 kDa protein, may play a critical regulatory role in replication based on its functioning during both the initiation and elongation stages of DNA replication. The protein is highly conserved, having been identified in a range of species from yeast (Saccharomyces cerevisiae) to humans. The primary function of RPA appears to be as an auxiliary protein for both DNA polymerase alpha and Δ. RPA undergoes a cell cycle dependent phosphorylation, which is restricted to the p34 protein. In addition to existing as part of the multi-subunit structure, p34 has also been identified as being present in a free, unphosphorylated form.

ALT Names :

5031405K23Rik, 70kDa, AA589576, AW557552, Cb1-727, HSSB, MST075, p70Rpa1, REPA1, Replication Factor 1a, RF-A, RP-A, RPA32, RPA70, replication protein A1, 70kDa

Immunogen :

replication protein A purified from U293 cells

Specificity :

Recognizes the complexed (phosphorylated) and the ~34 kDa unphosphorylated subunit of replication protein A.

Package Size :

100 ug

Uses :

WB, IF, IP, IH(P)

Storage :

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 ug/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h.