Replication Protein A (Ab-2) Mouse mAb (RPA34-19) | EMD Millipore NA18-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Replication Protein A (Ab-2) Mouse mAb (RPA34-19)

catalog :

NA18-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; loading ...; fig 2b western blot; human; loading ...; fig 2c	Clarke T, Sanchez Bailon M, Chiang K, Reynolds J, Herrero Ruiz J, Bandeiras T, et al. PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination. Mol Cell. 2017;65:900-916.e7 pubmed publisher
western blot; human; 1:100; loading ...; fig 3c	Zhang Y, Lai J, Du Z, Gao J, Yang S, Gorityala S, et al. Targeting radioresistant breast cancer cells by single agent CHK1 inhibitor via enhancing replication stress. Oncotarget. 2016;7:34688-702 pubmed publisher
immunocytochemistry; human; fig 3	Nakajima N, Hagiwara Y, Oike T, Okayasu R, Murakami T, Nakano T, et al. Pre-exposure to ionizing radiation stimulates DNA double strand break end resection, promoting the use of homologous recombination repair. PLoS ONE. 2015;10:e0122582 pubmed publisher
immunocytochemistry; human; 1:50; fig 3	Yaglom J, McFarland C, Mirny L, Sherman M. Oncogene-triggered suppression of DNA repair leads to DNA instability in cancer. Oncotarget. 2014;5:8367-78 pubmed

product information

Catalog Number :

NA18-100UG

Category :

Antibodies

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Replication Protein A (Ab-2) Mouse mAb (RPA34-19)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

29935 (Hu)

Host Name :

Clone :

RPA34-19

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Replication Protein A (Ab-2) Mouse mAb (RPA34-19)

Cross Reactivity :

Hamster, Human

Background :

The replication system of a cell contains several essential components among which are replication factor A (RFA, RPA, or HeLa cell SSB), PCNA, and replication factor C (RFC) as well as several other proteins. RPA, a multi-subunit protein containing a 70 kDa, 34 kDa, and 11 kDa protein, may play a critical regulatory role in replication based on its functioning during both the initiation and elongation stages of DNA replication. The protein is highly conserved, having been identified in a range of species from yeast (Saccharomyces cerevisiae) to humans. The primary function of RPA appears to be as an auxiliary protein for both DNA polymerase alpha and Δ. RPA undergoes a cell cycle dependent phosphorylation, which is restricted to the p34 protein. In addition to existing as part of the multi-subunit structure, p34 has also been identified as being present in a free, unphosphorylated form.

ALT Names :

HSU24186, MGC120333, MGC120334, RPA34, replication protein A4, 34kDa

Immunogen :

replication protein A purified from U293 cells

Specificity :

Recognizes the ~34 kDa phosphorylated subunit and the nonphosphorylated RPA complex.

Package Size :

100 ug

Uses :

WB, IF, IP, IH(P)

Storage :

Do not freeze